FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005113572

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005113572
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences61888630
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGAAGCTATCTCGTATGC2736400.44214906679950744TruSeq Adapter, Index 19 (97% over 37bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC312950.041.107845
TCGTATG323850.039.67082244
CTCGTAT332550.038.5586643
TATCTCG331600.038.41670240
CGTCTGA348200.037.2698315
ATCGGAA360300.036.7482871
ACGTCTG352250.036.73844514
GAAGCTA361600.035.85088735
TCTCGTA356500.035.58366842
ATCTCGT364800.034.97738341
GCTATCT366800.034.85869238
CACGTCT374600.034.621513
TCGGAAG397550.033.9447752
ACACGTC386800.033.65712
AGCTATC383650.033.45069537
CTATCTC384750.033.22656239
CACACGT398500.032.77033611
AAGCTAT401350.032.1770136
GCACACG418000.031.42436810
CGGAAGA432450.030.8450723
Produced by FastQC (version 0.11.5)