FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005113574

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005113574
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences64698157
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG51350.019.49790244
CGTATGC60550.016.60969545
AACGTTC65350.016.11270933
CGTATCG60600.015.8534413
GTATCGA59000.015.2156034
CCCGTAT78850.015.0496751
CTCGTAT71150.014.23003443
ATCGGAA80700.013.7001751
TATCGAA68800.013.6369085
CACAACG77250.013.572371530
CCGTATC80350.013.4497452
ACACGTC84050.012.42077112
CTCCGAT86450.012.1117281
TCGGAAG119500.011.6622272
ACGTCTG89750.011.58179314
CAACGTT91900.011.26187232
TCTCGTA90200.010.9004242
CGCTAGT9800.010.569391
ATCGAAG108350.010.3619126
CGTCTGA99900.010.2248915
Produced by FastQC (version 0.11.5)