FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005113581

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005113581
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences67982223
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACAGCGATAGATCTCGTATGC1534200.2256766449075959TruSeq Adapter, Index 1 (97% over 35bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC194650.038.85474445
TCGTATG198450.038.0894944
CGATAGA213950.035.7739735
AGCGATA213600.035.73790433
GCGATAG214200.035.67976434
CTCGTAT219250.034.39399743
ATCGGAA230300.034.281131
ACACGTC224900.033.91464212
ACGTCTG227600.033.5415214
CGTCTGA232200.032.97457515
TCTCGTA229800.032.22812742
ATCTCGT237550.031.11992541
CACACGT248450.030.83563211
CACGTCT253150.030.15833313
ATAGATC259250.028.92494837
CGGAAGA284600.027.998753
CACAGCG283500.027.06138830
TAGATCT282500.026.75917438
GATCTCG278900.026.67754240
GCACACG292850.026.46749310
Produced by FastQC (version 0.11.5)