FastQCFastQC Report
Thu 16 May 2024
EGAF00005114339

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005114339
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences63917348
Total Bases6.4 Gbp
Sequences flagged as poor quality0
Sequence length101
%GC53

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTCAGAGCCGTGTAGATCTC2544170.3980406070664884Illumina Single End Sequencing Primer (100% over 31bp)
CCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCT1483080.23203090340982233No Hit
CCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAG828160.12956732810629126No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)