FastQCFastQC Report
Thu 2 May 2024
EGAF00005114365

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005114365
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences58971029
Total Bases5.9 Gbp
Sequences flagged as poor quality0
Sequence length101
%GC54

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCT946040.16042453659745365No Hit
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCTTCGCCTGTGTAGATCTC881700.1495140944547534Illumina Single End Sequencing Primer (100% over 31bp)
CCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAG634050.10751889711810862No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)