FastQCFastQC Report
Thu 16 May 2024
EGAF00005114505

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005114505
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences67582560
Total Bases6.8 Gbp
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGATAGGGTGTAGATCTC3254510.4815606274754907Illumina Single End PCR Primer 1 (96% over 33bp)
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGATAGGGTGTAGATCT1028380.15216647608495446Illumina Single End PCR Primer 1 (97% over 34bp)
GACAAATCAAGAAACAAACTGCACTTGTTGAGCTTGTGAAACACAAGCCC1019830.15090135679974243No Hit

[OK]Adapter Content

Adapter graph