FastQCFastQC Report
Tue 12 Jul 2022
EGAF00005114528

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005114528
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences66489923
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCTCGTATG3160250.47529758757578944TruSeq Adapter, Index 6 (97% over 35bp)
CTCCTCGGCAAAGCAGGTCTCCTTATCGTCAGCCTTGCAGCACTTCTCTA1065610.16026639104394813No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCTCGTAT1030400.1549708517484672TruSeq Adapter, Index 6 (97% over 36bp)
CTCTTTTGTTGCCTTGGGCTTGTGTTTCACAAGCTCAACAAGTGCAGTTT852220.12817280597542577No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)