FastQCFastQC Report
Fri 24 May 2024
EGAF00005115251

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005115251
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences84020648
Total Bases8.4 Gbp
Sequences flagged as poor quality0
Sequence length101
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCTTATCTCGTA13652731.6249255778174907TruSeq Adapter, Index 20 (97% over 44bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCTTATCTCGTAT9908171.1792541757116657TruSeq Adapter, Index 20 (97% over 44bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)