FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005179458

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005179458
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36866721
Sequences flagged as poor quality0
Sequence length8
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGTACAC1028642427.901651465016375No Hit
ATTGCGTG1005277927.267895617839187No Hit
CGACTTGA789367921.411394303279643No Hit
TACAGACT685599618.59670676977212No Hit
CGCCTTGA1688100.457892634389698No Hit
TACAGCCT1629610.44202737748225557No Hit
GCGTACCC1217110.3301378497968398No Hit
GCGTACAA889380.24124195910995178No Hit
CGACTTGC746730.20254852608128615No Hit
GCGTCCAC599450.16259921786914547No Hit
TACAGAAT494350.13409112245160074No Hit
GCGTAAAC490650.13308750729418No Hit
ATTGCGGG446340.12106853766571754No Hit
CGACTTAA429770.11657396924451187No Hit
TAAAGACT421030.11420326749428027No Hit
ATTGAGTG395870.10737868442381951RNA PCR Primer, Index 32 (100% over 8bp)
ATTGCGTT374230.10150889198960765No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)