FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005179471

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005179471
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28785742
Sequences flagged as poor quality0
Sequence length8
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCGTCAC767286026.65507111124667No Hit
GATCAGCA681116823.661603025553415No Hit
TGGTGTTT674986023.448622585445253No Hit
ACAACAGG572488219.88790839576065No Hit
CTCGTCCC1210400.4204859475222143No Hit
TGGTGTTA1082680.37611675947071294No Hit
ACACCAGG932130.32381656168529543No Hit
CTCGTCAA894750.31083096624710943No Hit
GCTCAGCA859330.29852626345362226No Hit
CCAACAGG818300.2842726791617878No Hit
GATCAGAA781170.27137393227522155No Hit
CTAGTCAC772840.2684801385352512No Hit
GATCCGCA692750.24065733653834595No Hit
CTCGTAAC537700.18679386482377283No Hit
GATCAGCC491950.17090057987735732No Hit
TGGTGGTT433860.15072045042299065No Hit
ACCACAGG420540.14609315959269004No Hit
TGGGGTTT366820.1274311428206367No Hit
GATAAGCA364100.12648623057901373No Hit
ATCGTCAC327730.11385150328937152No Hit
ACAAAAGG322030.11187135631244108No Hit
ACAACAGT303170.10531950157824661No Hit
TGGTTTTT291170.1011507711004983No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)