FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005179481

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005179481
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37037083
Sequences flagged as poor quality0
Sequence length8
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTCAACC1141384330.817337855683718No Hit
AGCGCCTA1080454429.172232597259345No Hit
CAAAGTGG710027419.170716009141433ABI Solid3 EF1 alpha Antisense Primer (100% over 8bp)
TTGTTGAT660166017.82445987984529No Hit
GCTCACCC3044740.8220787798002344No Hit
CGCGCCTA754190.2036310472938703No Hit
GCTCCACC492740.1330396348978131No Hit
AGCGCCGA414210.11183656121082754No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)