FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005179491

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005179491
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25623390
Sequences flagged as poor quality0
Sequence length8
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCATTCC731834728.561197405963846No Hit
TTGCCGGA638575924.9216009279022No Hit
AGTTGCAG526698020.555359770896825No Hit
CAAGAATT524569420.472287234436973No Hit
GCCATTCA1759580.6867085112469505No Hit
GCCATTAC830820.3242428109629522No Hit
AAAGAATT538280.21007368658089348No Hit
GACATTCC471280.18392570225875654No Hit
GCAATTCC463010.18069818240287486No Hit
TTGCCGGC460690.17979275966216804No Hit
TTGACGGA433770.16928673372258704No Hit
TTGCCTGA396870.15488582892427583No Hit
GCCCTTCC382530.1492893797424931No Hit
GCCATACC357630.13957169601680341No Hit
CAAGAATA351030.13699592442686157No Hit
TTGCCGAA332130.12961985123748263No Hit
AGTTGAAG324460.12662649243523202No Hit
CAATAATT314250.12264185183927653No Hit
TTGCAGGA300910.11743567108021226No Hit
CAAGAAAT293150.114407188119917No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)