FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005179497

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005179497
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19463932
Sequences flagged as poor quality0
Sequence length98
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG529310.2719440244653547No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA108700.032.5416958
TGGTATC120650.029.6227077
GTGGTAT125250.028.3871426
GTATCAA133650.026.5713069
ATCAACG80250.025.0507710-11
TCAACGC80400.021.8864412-13
AAGCAGT174000.021.307831
CAGTGGT174350.020.946844
CAACGCA99500.020.62108612-13
AGAGTAC130800.015.84486318-19
GAGTACA114700.015.4222320-21
CGCAGAG115850.015.22899616-17
GTACATG129450.014.10906422-23
TACATGG151000.013.8778222-23
AGTACAT154250.013.39169120-21
GCAGAGT161750.012.88415316-17
ACGACGC8400.012.594015
CAGAGTA152450.012.26689218-19
TCTCCCG36750.011.263453592
CGACGCT10250.011.21853356
Produced by FastQC (version 0.11.5)