FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005179498

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005179498
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32019999
Sequences flagged as poor quality0
Sequence length26
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCGAT95350.017.7403431
CATGGCG143000.017.5230921
AGCTTGA99850.017.371611
GGCGACT91200.017.3191411
TTGAACG120600.017.2775061
CCTACCA142300.017.229691
TGATTTC86850.017.1269571
CAATGGA126050.017.1262518
TATTGTC79850.017.0874463
CTGATAG117800.016.9663831
TGCTACC109200.016.965141
GGATTAC108600.016.9114911
ATGGCGC90400.016.9079362
CGCTATC113800.016.8683191
ACTGCTC104250.016.849521
CTGAAAC132600.016.8379691
CGCTTCA102250.016.8366871
TGACGGC100400.016.8280981
AGCGTAT110700.016.8165861
ACACCCT119700.016.7973581
Produced by FastQC (version 0.11.5)