FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005179510

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005179510
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32320659
Sequences flagged as poor quality0
Sequence length26
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGG390840.12092575216365482No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAATGGA113050.016.9480578
ACAGCCG128250.016.804071
CTGCGGA88400.016.7204021
CGGACAC82750.016.701851
GATGCTA97900.016.6301541
GGGATGA108700.016.6246761
CTGATCC101450.016.6101071
GGGAGAT68800.016.5997751
CGACCTT115300.016.592441
ATCGAGT91400.016.576431
ACCCACT119500.016.5699751
CGAGCAC118200.016.549161
CCGAATT99000.016.52506310
ACCTTTA146550.016.5004221
CGACTTC126600.016.4701251
GACTAAC78700.016.468531
ACGATGT132600.016.4338111
ATGCGAT67700.016.4263341
CCGTTCA110200.016.371161
CGCTTCA101600.016.3591921
Produced by FastQC (version 0.11.5)