FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005179512

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005179512
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33635015
Sequences flagged as poor quality0
Sequence length26
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACTAAC271750.019.0972881
GGCAGAG118450.018.7332043
GTCTGAG223400.018.6298223
GGAACCA176800.018.6194743
TTGGAAC200950.018.569271
CGGAGGC107750.018.5239815
ACGGAGA120100.018.5203571
ACTGAGA125150.018.5133764
GGGACCT144350.018.4991631
GGCGACT114100.018.4687061
GAAGATG142000.018.4572036
AAAGGCG92550.018.400359
AGAAACC175100.018.3550138
GAAGAAA111000.018.3327946
AGGGTGA88950.018.3273331
CGGACTG128250.018.2948231
CGGACGT134250.018.2445051
TACGGTA228300.018.2152941
GGAGGCT109600.018.1928966
TGAGGCA186200.018.1681026
Produced by FastQC (version 0.11.5)