FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005179528

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005179528
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27459436
Sequences flagged as poor quality0
Sequence length26
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGG906440.3301014631181791No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAATGGA90700.017.0882578
GGGATGA100100.016.8862021
GACTAAC65150.016.825791
CTGATCC84100.016.816391
ATGCGAT59700.016.7869851
ATCGAGT71250.016.6626931
CTGCGGA83700.016.6577631
GGGAGAT65450.016.6264131
GATCGCG65550.016.601051
CGACCTT91500.016.549471
CGAGCAC107200.016.523541
CCGAATT84350.016.5133910
ACCTTTA119800.016.497171
ATTGGTG92250.016.4908181
CGCTGGA80000.016.4280011
ACAGCCG95950.016.4177991
AGGCCAC99900.016.3793681
CGGACAC69700.016.3731481
CTGATAG72950.016.3429431
CGCTTCA93700.016.3316921
Produced by FastQC (version 0.11.5)