FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005179533

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005179533
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20922082
Sequences flagged as poor quality0
Sequence length98
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG756360.3615127786995577No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA124250.036.2053458
TGGTATC135950.033.156357
GTGGTAT144750.031.2034326
GTATCAA147600.030.3541019
ATCAACG94750.026.60566910-11
CAGTGGT190150.024.379214
AAGCAGT191900.023.667791
TCAACGC93650.023.58002312-13
CAACGCA115750.021.6812612-13
CGCAGAG130050.017.29975316-17
GAGTACA130250.017.0427320-21
AGAGTAC149750.016.91226618-19
TACATGG165400.015.39585422-23
GTACATG148750.015.24795522-23
AGTACAT173350.014.78260420-21
GCAGAGT188250.013.74766516-17
CAGAGTA175850.013.06785818-19
AGTGGTA358900.012.91760355
TATCAAC191250.012.44748910-11
AGCAGTG432350.011.6430252
Produced by FastQC (version 0.11.5)