FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005179534

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005179534
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21447882
Sequences flagged as poor quality0
Sequence length26
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGG448670.20919081893494193No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCGAT60250.017.4968931
GGGAATG86350.017.4900881
GGGAGAT90800.017.4370271
CGATTGA84950.017.4251171
ACTGAAC56850.017.0654851
CGTCTAC84300.017.0611651
CAATGGA82850.017.0302018
GGAACTT72850.017.010581
CACGAGT72400.016.9627253
TGATTTC83300.016.833731
CCTTTCT178250.016.8108481
CGTTGAC68750.016.7994279
CCTCTGA79400.016.7788071
TCCCGAT70250.016.7574331
GGCGCTC49750.016.7431499
CTAAGAC71000.016.7353761
ATGCGAT50900.016.7220341
GATGCTA88850.016.716541
CTAGCCT97350.016.6028561
CACCGAA47300.016.5956298
Produced by FastQC (version 0.11.5)