FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005179541

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005179541
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19917317
Sequences flagged as poor quality0
Sequence length98
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG646990.32483792872303036No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA107900.032.7417378
TGGTATC119950.029.911627
GTGGTAT127900.028.0519546
GTATCAA135600.026.0877939
ATCAACG82350.024.77475710-11
TCAACGC82700.021.36075612-13
CAGTGGT174650.021.1742974
AAGCAGT176850.020.7561451
CAACGCA104750.019.74088112-13
AGAGTAC131900.015.6950618-19
GAGTACA114500.015.40777220-21
CGCAGAG116150.015.407516-17
TACATGG149200.013.905498522-23
AGTACAT156150.013.34554220-21
GTACATG135100.013.31374622-23
GCAGAGT168950.012.66183216-17
CAGAGTA155800.011.70768318-19
AGTGGTA342850.010.7732075
AGCAGTG380200.010.621812
TATCAAC176200.010.612910-11
Produced by FastQC (version 0.11.5)