FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005179554

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005179554
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22564869
Sequences flagged as poor quality0
Sequence length26
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGG1119290.49603212852687073No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAATGAA136200.017.9182851
CGGCTAG109000.017.7648941
GGGACCT62450.017.6014731
GACTAAC73850.017.4982951
TGCCAAA108100.017.3854061
AAACCTG74750.017.2474731
TATGCCC80400.017.242131
TACGGAT79600.017.1012861
CCGGTAG65300.016.9251631
CTGCGGA58700.016.8686711
TCCACGA69100.016.8149418
GATGCTA71650.016.765271
AGAAACC71850.016.755878
ACTGAAC59350.016.7344821
CGCTGGA80350.016.7175981
GATCGCG63050.016.6725231
CAATGGA77150.016.6675728
AGACGTT66850.016.6524261
GGGAATG64200.016.5919841
GTTTGAC77300.016.5317428
Produced by FastQC (version 0.11.5)