FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005179555

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005179555
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22564869
Sequences flagged as poor quality0
Sequence length98
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT7457723.3050136475421157No Hit
GCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT773870.34295346452044545No Hit
GAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT467280.20708296600348092No Hit
CAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT333450.14777395782798472No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT330260.14636025584726417No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTA310030.13739499218896417No Hit
AGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT235120.10419736981411237No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT1495250.083.6946641
AGCAGTG1647100.079.8283842
GCAGTGG1915450.069.177253
CAGTGGT1949150.067.381374
AGTGGTA1944650.067.1214455
GTGGTAT2055150.063.8581476
TGGTATC2062750.063.8291367
GGTATCA2100750.062.401698
GTATCAA2103750.062.2184079
GAGCAGT195750.045.5204621
ATCAACG2131150.032.49716610-11
TAGTATC89550.032.153547
CAACGCA2153050.032.14863612-13
ACGCAGA2122350.031.94980614-15
AGAGTAC2165050.031.55852318-19
GCAGAGT2170800.031.45608316-17
TCAACGC2152500.031.28815312-13
AGTACTT2156350.031.06596820-21
AACGCAG2148500.030.95821814-15
CGCAGAG2184450.030.65198316-17
Produced by FastQC (version 0.11.5)