FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005179559

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005179559
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27092793
Sequences flagged as poor quality0
Sequence length98
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2349200.8670940644620878No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG987500.36448807622012247No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC689500.25449572511774626No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT1005900.072.470291
AGCAGTG1126550.064.675712
GTGGTAT1288750.056.3487636
CAGTGGT1312850.055.516444
TGGTATC1331150.054.5850337
GTATCAA1349450.053.774849
GGTATCA1357200.053.5831958
AGTGGTA1448600.050.257435
GCAGTGG1535750.047.682493
CGCAGAT302800.034.71397892
CAACGCA1286800.031.15084812-13
ATCAACG1289750.031.1221610-11
TACATGG1356100.029.31800522-23
AGTACAT1385500.028.89127320-21
AGAGTAC1394500.028.76124618-19
GCAGAGT1398150.028.70930316-17
TCAACGC1280300.028.43316812-13
GAGTACA1332500.027.29895620-21
CGCAGAG1349750.027.12308516-17
TATCAAC1347350.027.08799210-11
Produced by FastQC (version 0.11.5)