FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005179565

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005179565
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26872896
Sequences flagged as poor quality0
Sequence length98
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2414020.8983103272531551No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG977280.36366754070718693No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC692950.2578620480650839No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT1001350.072.413561
AGCAGTG1119700.064.700422
GTGGTAT1276450.056.6139346
CAGTGGT1298950.055.8418734
TGGTATC1318800.054.834277
GTATCAA1331750.054.2764669
GGTATCA1340150.053.967478
AGTGGTA1436250.050.4219475
GCAGTGG1523900.047.8773043
CGCAGAT301600.033.64475392
CAACGCA1271150.031.3139212-13
ATCAACG1276550.031.16579410-11
TACATGG1340600.029.45373522-23
AGTACAT1373100.028.96291220-21
GCAGAGT1386950.028.78248816-17
AGAGTAC1386100.028.75860218-19
TCAACGC1266700.028.63899812-13
GAGTACA1319900.027.5139820-21
CGCAGAG1331850.027.363816-17
TATCAAC1335850.027.18281610-11
Produced by FastQC (version 0.11.5)