FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005179572

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005179572
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46596988
Sequences flagged as poor quality0
Sequence length8
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGATGGG1284761927.57177996140008No Hit
GTCTCCTT1271623527.28982182281825No Hit
TCACATCA983455521.1055594408806Illumina PCR Primer Index 1 (100% over 8bp)
AGTGGAAC966495320.741583125501588No Hit
AGTGGCAC2096400.4499003240295274No Hit
TCACCTCA1091390.23421900145133845RNA PCR Primer, Index 40 (100% over 8bp)
TCCCATCA835930.17939571544838906No Hit
GGCTCCTT576920.12381057762789302No Hit
GTCTCCGT512430.10997062728603832No Hit
CAGCTGGG491520.10548321277761559No Hit
AGTGGACC483890.10384576788525474No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)