FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005179588

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005179588
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25144820
Sequences flagged as poor quality0
Sequence length98
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT7772603.091133680813782No Hit
GCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT1223470.4865694007751895No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT624890.24851639423149577No Hit
CAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT425230.1691123658868904No Hit
AGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT355100.14122192960617733No Hit
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT316220.12575950036627823No Hit
CTTAGCTTTGGCTCTCCTTGCAAAGTTATTTCTAGTTAATTCATTATGCA260930.10377087606910687No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT1209400.081.364091
AGCAGTG1243700.079.164282
CAGTGGT1468500.067.19754
GCAGTGG1506900.066.078563
AGTGGTA1642800.060.8779035
TGGTATC1705750.057.5329447
GTGGTAT1723900.057.4142466
GTATCAA1741750.056.6454289
GGTATCA1749000.056.4289178
ATCAACG1732050.029.8128810-11
CAACGCA1755050.029.60776312-13
ACGCAGA1785200.029.0460514-15
AGAGTAC1791050.028.79139918-19
TCAACGC1733900.028.5562912-13
GCAGAGT1823950.028.4625516-17
AGTACTT1801350.028.29094320-21
AACGCAG1782550.028.02881214-15
TACTTTT1869600.027.50065422-23
GTACTTT1788450.027.47937222-23
CGCAGAG1817050.027.4696216-17
Produced by FastQC (version 0.11.5)