FastQCFastQC Report
Wed 10 Jul 2024
EGAF00005179601

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005179601
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29116950
Total Bases232.9 Mbp
Sequences flagged as poor quality0
Sequence length8
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGAGTCGC1060468936.42101593745224No Hit
TATCGATA697195823.944671402739644No Hit
GTGTCGCT591096320.300762957658684No Hit
CCCAATAG452509015.541085175473393No Hit
AGCGTCGC977270.3356361157332756No Hit
AGAGTCGA603180.20715768650219202No Hit
AGAGTCTC546900.18782873893041682No Hit
AGAGTAGC476350.16359886595264958No Hit
TATCGAAA368080.12641433941398397No Hit
TATCTATA362690.12456318398733383No Hit
CCAAATAG314060.10786157203965388No Hit
CCCAATAT312360.10727771967874382No Hit
GTGTCGCG302410.10386046615459381No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.12.1)