FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005179604

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005179604
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32328541
Sequences flagged as poor quality0
Sequence length26
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTGAAC117400.017.8680551
CCCGACA137050.017.66475110
TATGCCC118650.017.5449161
TGAGCAT135600.017.4173971
TCGAATG123500.017.4165948
TACGGAT119350.017.316291
TCGCTAG106150.017.3147138
CCACTAC130650.017.2503811
ACTGATG101550.017.219021
AGCTACC99300.017.1999978
CAATGGA118950.017.1412538
GCTGCGA164750.017.1226371
CCTTACG119150.017.0515121
CAGATGT196800.017.0217977
CTGCGAT94700.016.9799292
TGGTTCG160800.016.971444
CGATTGA97700.016.9658221
CTCGAAA93250.016.9279791
TCCGCAC104650.016.9231244
CTGATAG111450.016.9191381
Produced by FastQC (version 0.11.5)