FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005179606

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005179606
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences54911372
Sequences flagged as poor quality0
Sequence length8
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAGTGCT1774848132.32204979325594No Hit
CTGCAAGC1248969022.745179268148682No Hit
GCTACCTG1238357022.55192239596563No Hit
TGCTGTAA1084500819.75002190803027No Hit
GCTCCCTG1054850.1921004632701583No Hit
CTGCACGC819320.14920770874200703No Hit
AAAGTGGT731660.13324380239488462ABI Solid3 EF1 alpha Antisense Primer (100% over 8bp)
CTGCCAGC610410.11116276606601636No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)