FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005179609

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005179609
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences55576119
Sequences flagged as poor quality0
Sequence length8
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAGTGCT1794428132.28775474588285No Hit
CTGCAAGC1259830622.668560213785348No Hit
GCTACCTG1252393222.534736547544817No Hit
TGCTGTAA1099933119.791470145657346No Hit
GCTCCCTG1070100.19254673036812808No Hit
CTGCACGC855690.15396721026885665No Hit
AAAGTGGT754510.13576154894874903ABI Solid3 EF1 alpha Antisense Primer (100% over 8bp)
CTGCCAGC626200.11267429451128101No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)