FastQCFastQC Report
Wed 10 Jul 2024
EGAF00005179620

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005179620
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences39620791
Total Bases316.9 Mbp
Sequences flagged as poor quality0
Sequence length8
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTCAACC1237196231.22593387900812No Hit
AGCGCCTA1169761529.523931008848358No Hit
CAAAGTGG756837919.102039128900785ABI Solid3 EF1 alpha Antisense Primer (100% over 8bp)
TTGTTGAT701982017.717516038485957No Hit
GCTCACCC2734100.6900669903334338No Hit
CGCGCCTA586090.14792486096504232No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.12.1)