FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005179621

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005179621
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37797017
Sequences flagged as poor quality0
Sequence length8
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTCAACC1164979430.82199317475239No Hit
AGCGCCTA1106157429.265732795791795No Hit
CAAAGTGG723944019.153469174564755ABI Solid3 EF1 alpha Antisense Primer (100% over 8bp)
TTGTTGAT672168017.783625623154332No Hit
GCTCACCC3150720.8335895925331885No Hit
CGCGCCTA794270.21014092196746637No Hit
GCTCCACC493390.13053675637947829No Hit
AGCGCCGA385550.10200540428891516No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)