FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005179622

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005179622
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37849324
Sequences flagged as poor quality0
Sequence length8
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTCAACC1171583530.953881765497314No Hit
AGCGCCTA1108619229.290330257945957No Hit
CAAAGTGG722122819.078882359959717ABI Solid3 EF1 alpha Antisense Primer (100% over 8bp)
TTGTTGAT671961617.753595810588322No Hit
GCTCACCC3178650.8398168485122747No Hit
CGCGCCTA636920.16827777426090887No Hit
GCTCCACC490910.1297011275551447No Hit
AGCGCCGA420130.11100066146491812No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)