FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005179624

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005179624
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32320659
Sequences flagged as poor quality0
Sequence length8
%GC52

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATACGCC1414637943.76884456471014No Hit
CTAGGTGA913984028.278631323699187RNA PCR Primer, Index 38 (100% over 8bp)
AGCCAATT753191223.303708009171473No Hit
TCGTTCAG1933690.5982829743663334No Hit
GATACGCA1707650.5283462815532319No Hit
CTAGGTGC781690.24185459832362946No Hit
GATACGAC530700.16419838469258935No Hit
GATACTCC511010.15810630593887334No Hit
ATAGGTGA441990.13675154333950926RNA PCR Primer, Index 39 (100% over 8bp)
GATCCGCC423190.1309348302582568No Hit
AGCCAATA391940.12126609175883449No Hit
GATAAGCC388970.1203471748518494No Hit
AGCAAATT368880.11413133624534079No Hit
GCTACGCC364570.11279782383150046No Hit
GAAACGCC362220.11207073469634392No Hit
GATACCCC361230.11176442906068221No Hit
AGACAATT352700.10912525019987988No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)