FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005179625

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005179625
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences111809075
Sequences flagged as poor quality0
Sequence length8
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CACCGCTC3034847327.143121432674405No Hit
GTTGAGAA2960580326.47889091292455No Hit
TCGATACT2621145223.443045208986838No Hit
AGATCTGG2352481921.04016959267394No Hit
AGATCTGA3421940.30605208029849096Illumina PCR Primer Index 9 (100% over 8bp)
CACCGCTA3395120.3036533483529848No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)