FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005179626

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005179626
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27445797
Sequences flagged as poor quality0
Sequence length8
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGCTCTC918570533.46853071892939No Hit
TTCGCTGA688660025.091637892679884No Hit
ACAAGAAT513420418.706703980941054No Hit
CATTAGCG492709617.952096636144326No Hit
TTCGCTGC1158860.4222358709422795No Hit
GGGCTCTA762630.27786768225386205No Hit
GGGCTATC564580.20570727095299873No Hit
GGGCTCGC483400.17612897158716143No Hit
GGGCGCTC480380.1750286209578829No Hit
TTCGCGGA424910.15481787612143308No Hit
TTCGCTTA315240.11485911668005122No Hit
TTAGCTGA312570.11388629013032486No Hit
CCAAGAAT301890.10999498393141943No Hit
ACAAGAAA298570.1087853269482391No Hit
GGGCTGTC290790.10595064883705144No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)