FastQCFastQC Report
Wed 10 Jul 2024
EGAF00005179637

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005179637
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24533768
Total Bases196.2 Mbp
Sequences flagged as poor quality0
Sequence length8
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGAGTCGC872656635.56961164709799No Hit
TATCGATA585199823.852830107466573No Hit
GTGTCGCT491768020.044536167457032No Hit
CCCAATAG375785915.31708867549412No Hit
AGCGTCGC1228030.5005468381375417No Hit
AGAGTCGA674760.27503317060795557No Hit
AGAGTCTC651310.2654749160422484No Hit
AGAGTAGC552750.22530171476309713No Hit
TATCTATA427850.17439229065832856No Hit
TATCGAAA384400.15668200661227413No Hit
GTGTCGCG378260.15417933356180755No Hit
CCAAATAG376700.15354347526234047No Hit
CCCAATAT351870.14342273066248934No Hit
TATAGATA329370.1342516974970987No Hit
ATAGTCGC302930.12347471452407961No Hit
GTGTCGCA278300.11343549021903199No Hit
AGAGACGC269410.10981191311501763No Hit
GGGTCGCT263300.10732146810877156No Hit
AGATTCGC258680.10543834929881134No Hit
GTGTCGAT255480.10413402458195578No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.12.1)