FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005179644

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005179644
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33392183
Sequences flagged as poor quality0
Sequence length8
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATACGCC1465734243.89453064509139No Hit
CTAGGTGA951288628.488362081628505RNA PCR Primer, Index 38 (100% over 8bp)
AGCCAATT782032423.419624886459207No Hit
TCGTTCAG2027030.6070372817494442No Hit
GATACGCA1526630.45718185001561595No Hit
CTAGGTGC791210.23694467654301007No Hit
GATACGAC466490.1397003604106985No Hit
GATACTCC452120.13539695802457719No Hit
ATAGGTGA416610.12476273264314586RNA PCR Primer, Index 39 (100% over 8bp)
GATCCGCC410550.1229479366473285No Hit
AGCCAATA377050.11291564855163856No Hit
GCTACGCC346750.10384166857255184No Hit
GAAACGCC344430.10314689518801451No Hit
GATAAGCC334230.10009228806634177No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)