FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005179661

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005179661
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26985234
Sequences flagged as poor quality0
Sequence length98
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT3562031.319992259470494No Hit
GCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT367660.13624488118205683No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCAGTG721450.072.669832
AAGCAGT766350.068.5665361
AGTGGTA840250.064.0921555
CAGTGGT814050.064.040864
GTATCAA820400.063.5623979
GGTATCA832700.062.419348
GTGGTAT854550.061.92176
TGGTATC844900.061.5285727
GCAGTGG955400.055.358843
ATCAACG803050.033.78225710-11
CAACGCA820400.033.24139412-13
AGAGTAC833350.032.59379618-19
TCAACGC805750.032.5298612-13
ACGCAGA849150.032.2782814-15
AACGCAG848350.031.26501314-15
TATCAAC854200.030.86540610-11
GTACTTT840950.030.52237322-23
GCAGAGT905150.030.21708916-17
AGTACTT886950.029.79719720-21
GAGTACT859550.029.75175320-21
Produced by FastQC (version 0.11.5)