FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005179675

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005179675
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24533768
Sequences flagged as poor quality0
Sequence length26
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGG433180.1765648065148411No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTTGCG151700.018.5795291
TATTACA237800.018.4622693
GACTGCG179600.018.4554861
GGGCACT213150.018.4551641
CCTATTA259450.018.4308091
GGGAGTT169000.018.3742642
CTATTAC243750.018.3071172
ATTACAC193750.018.3033374
ACCCTAT186100.018.2583169
AGCATCG145550.018.1052066
TTACACC190550.018.0648845
TGTACCG138000.018.0356667
ACCCGAG95950.018.0087410
CCGTGGA94600.018.0054021
GGCCCGT115450.017.9984599
CGACTTC139950.017.988281
CTCTACG124150.017.9090291
TCGCCAA130450.017.8990158
CACCCTA190750.017.8605168
CGCTGGA110600.017.8423181
Produced by FastQC (version 0.11.5)