FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005179676

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005179676
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24533768
Sequences flagged as poor quality0
Sequence length98
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT12324385.0234354543501025No Hit
GCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT2301680.938168160716283No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1255950.5119270712921065No Hit
AGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT581660.23708547337693905No Hit
CAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT566820.23103666750252144No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTA472760.1926976728564483No Hit
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT453320.18477390020155077No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG304490.1241105728235467No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTAA272430.11104286956655007No Hit
CTTAGCTTTGGCTCTCCTTGCAAAGTTATTTCTAGTTAATTCATTATGCA263990.10760271312584353No Hit
GTTTCGGGGGTCTTAGCTTTGGCTCTCCTTGCAAAGTTATTTCTAGTTAA263050.10721956774026721No Hit
GCTTTGGCTCTCCTTGCAAAGTTATTTCTAGTTAATTCATTATGCAGAAG247520.10088951684877757No Hit
GGTAGCTCGTCTGGTTTCGGGGGTCTTAGCTTTGGCTCTCCTTGCAAAGT246680.10054713161060298No Hit
GTCTGGTTTCGGGGGTCTTAGCTTTGGCTCTCCTTGCAAAGTTATTTCTA245400.10002540172386076No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT2024800.086.935111
AGCAGTG2090500.084.130212
GCAGTGG2596950.068.1855243
CAGTGGT2683550.065.3496554
AGTGGTA3030100.059.6628535
TGGTATC3205300.054.6131137
GTGGTAT3287900.054.249126
GGTATCA3334700.052.5480238
GTATCAA3348050.052.305529
AAGTGGT161600.036.034974
AAGAAGT215350.030.8394891
AGAAGTG215950.029.5200422
ATCAACG3362850.027.1869510-11
CAACGCA3383000.027.07706312-13
ACGCAGA3418900.026.98690614-15
GCAGAGT3457250.026.66873416-17
AGAGTAC3455200.026.53143918-19
AGTACTT3430600.026.4153920-21
TCAACGC3360950.026.05921712-13
TACTTTT3565300.025.90279622-23
Produced by FastQC (version 0.11.5)