FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005179683

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005179683
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences59718735
Sequences flagged as poor quality0
Sequence length8
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAGTGCT1919501132.142360349729444No Hit
CTGCAAGC1370952822.95682920945998No Hit
GCTACCTG1354814522.686590732372345No Hit
TGCTGTAA1182079019.79410648936217No Hit
GCTCCCTG988400.16550919908132683No Hit
CTGCACGC813540.13622860564611758No Hit
AAAGTGGT740440.12398789090224366ABI Solid3 EF1 alpha Antisense Primer (100% over 8bp)

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)