FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005179684

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005179684
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences57137418
Sequences flagged as poor quality0
Sequence length8
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAGTGCT1838700132.1803148332674No Hit
CTGCAAGC1301252322.77408300109046No Hit
GCTACCTG1290607322.58777776762681No Hit
TGCTGTAA1130543919.78640161863807No Hit
GCTCCCTG1083050.18955179248736792No Hit
CTGCACGC915430.1602155001123782No Hit
AAAGTGGT796370.13937801669651925ABI Solid3 EF1 alpha Antisense Primer (100% over 8bp)
CTGCCAGC647780.11337229134155133No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)