FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005179689

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005179689
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37910025
Sequences flagged as poor quality0
Sequence length26
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTCGTTATCGGAATTAACCAGACA389530.10275118520760669No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTGGA182500.017.7918471
CTCTACG217650.017.7725981
CAATGGA199100.017.7542958
GACTAAC120950.017.6491551
ATGCGAT127150.017.5674111
TGACAAC152400.017.3676171
ACTGATG108700.017.3467141
CGGACAC137750.017.2394941
GGGCATC157100.017.1854951
CGGACTG87700.017.1433121
TGACTAG125200.017.0660211
TGCGGGT140400.017.0565991
CGTTGGG186450.016.8837831
CCCTCCT200000.016.875261
CAACTAG164950.016.8467181
TCCCGAT123350.016.8110771
CGCTTCA136050.016.8005311
AAACTAG122850.016.7272383
TATATGA90700.016.7138989
CTTGGCT159500.016.7136731
Produced by FastQC (version 0.11.5)