FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005179691

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005179691
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences38700541
Sequences flagged as poor quality0
Sequence length26
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTCGTTATCGGAATTAACCAGACA394220.10186420908172833No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCTACG217850.017.9796831
GACTAAC121450.017.79731
CAATGGA199850.017.7304528
CCGTGGA190500.017.6994861
ATGCGAT124400.017.4878251
TGACAAC155850.017.4180661
CGGACAC141150.017.2620871
ACTGATG114250.017.2467371
TGCGGGT139850.017.2151371
GGGCATC161200.017.063361
TGACTAG126950.016.9789731
CCCTCCT203250.016.9730361
CAACTAG171300.016.944821
TCCCGAT121250.016.9439871
AAACTAG121350.016.9291923
CGTGAGC122900.016.7734741
CGGACTG90200.016.7332231
GTCGTAA165700.016.7327611
CGCTTCA140250.016.6953451
CTACCCA201950.016.6613061
Produced by FastQC (version 0.11.5)