FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005179694

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005179694
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24711280
Sequences flagged as poor quality0
Sequence length8
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGAGTCGC872972435.326879060898506No Hit
TATCGATA584698623.661202495378628No Hit
GTGTCGCT492155419.91622449342972No Hit
CCCAATAG376867515.250828771314154No Hit
AGCGTCGC1411710.5712816171400267No Hit
AGAGTCGA738150.2987097390341577No Hit
AGAGTCTC691600.27987218792389545No Hit
AGAGTAGC613680.2483400293307348No Hit
TATCTATA462230.18705222877973138No Hit
GTGTCGCG430090.17404602270703906No Hit
CCAAATAG416550.16856674360858684No Hit
TATCGAAA413530.16734462965900593No Hit
CCCAATAT378670.1532377116847043No Hit
GGGTCGCT374870.15169995241039722No Hit
ATAGTCGC354310.14337986538940922No Hit
TATAGATA352910.14281332249887502No Hit
AGAGACGC306100.12387055628037073No Hit
GTGTCGCA286030.11574875927106974No Hit
AGATTCGC278860.11284725032454815No Hit
GTGTCGAT268230.10854557109142059No Hit
TATCGCTA261810.10594756726482804No Hit
TTGTCGCT258630.10466070555632893No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)