FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005179699

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005179699
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29284740
Sequences flagged as poor quality0
Sequence length26
%GC53

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTACGA182500.018.952687
AAATGCC295750.018.8609751
AGAGCGA199600.018.736271
TTTAAGA138150.018.6894474
GTGCAGC292400.018.6802671
AGAAGAA104950.018.6652368
AGATGCA174550.018.6361433
AGAGCTT194400.018.6354731
AGTCTTT200850.018.6346051
AATGCCA206900.018.6034132
ATCGAGT179450.018.5994871
CGACCTT347000.018.5570851
GACTACA218500.018.5529691
TAAGAGT191150.018.5348016
AGACCTA167450.018.5301138
TCCAGAA134700.018.4698878
CTCCCTG208000.018.4222289
ATAGGAT133050.018.4207959
AGCAGAA113250.018.4196452
ACTGAAC122300.018.4093021
Produced by FastQC (version 0.11.5)