FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005179703

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005179703
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29969830
Sequences flagged as poor quality0
Sequence length26
%GC52

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGCAGC289000.018.9465561
CGTACGA182100.018.9227437
AAATGCC302900.018.9058441
ATCGAGT177700.018.758231
AGAGCTT201350.018.745381
AGAAGAA104950.018.7128818
GACTACA218450.018.6789341
AGAGCGA196950.018.6766821
TTTAAGA136550.018.6012064
CGACCTT366400.018.5908551
AGTCTTT206050.018.5798951
TAAGAGT189600.018.5337146
GAGAATT100400.018.524997
TTAAGAG138250.018.5099075
CTTAGGA153600.018.4979671
TATGAAA87900.018.452019
AGATGCA188650.018.4098053
CCAGAGA116150.018.3985844
GTCGAAA131100.018.3897678
AATGCCA215250.018.37962
Produced by FastQC (version 0.11.5)