FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005179714

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005179714
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31922291
Sequences flagged as poor quality0
Sequence length26
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCGAT94250.017.4192831
CTGATAG119900.017.4059681
CATGGCG140400.017.4012951
GGATTAC106550.017.3802491
TGATTTC89050.017.3579161
GGCGACT90100.017.1889441
AGCTTGA102550.017.1801411
CAATGGA126850.017.17568
CCTACCA144200.017.1023061
CGCTTCA103250.017.0830441
TTGAACG123500.017.0282021
TATTGTC80700.016.9190673
GCTTAAC89300.016.90713110
ATGGCGC88750.016.8838542
TGGGCGT86450.016.8615531
CTGAAAC129000.016.8528371
ACACCCT111500.016.8150351
AGCTTAA90050.016.7996549
CGCTATC109500.016.7566911
TGACGGC102350.016.7347371
Produced by FastQC (version 0.11.5)