FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005179724

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005179724
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31880397
Sequences flagged as poor quality0
Sequence length26
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATGGCG143900.017.5315971
GGCGACT88300.017.3440951
AGCTTGA102900.017.3232561
TTGAACG121100.017.2721771
TGATTTC85350.017.20521
GCGCGAT96000.017.1825081
CGCTATC108350.017.1350841
CAATGGA129300.017.1303928
GGATTAC106900.017.049351
CCTACCA141000.016.991181
CTGATAG113900.016.9763891
CCACTAC104850.016.9533751
TGGGCGT83650.016.944981
TGCTACC101200.016.922341
CTGAAAC132050.016.9004521
TGACGGC100800.016.8803311
TATTGTC79600.016.8726633
GCTTAAC86400.016.72394410
ACTGCTC103050.016.6767851
AAGCATT109800.016.6585625
Produced by FastQC (version 0.11.5)